They don't usually understand that the eggs they are laying are fertile or not fertile, so no, they don't get upset when you collect the eggs  
 
 
I'm hatching chicks & ducks at the moment, I've lost count of how many we have... hang on... I have 32 chicks of varying ages - between POL & 6 that are just 3 days old. Then there's the 23 ducklings, but a further 4 eggs are pipping now, so that'll make 27... then the muscovy duck is sitting on 13 eggs, due next week & another muscovy is hoarding eggs & plucking feathers, so she's due to go broody later this week too - phew!! 
Usually when they go broody, they will sit tight on an egg & try to peck at you if you disturb them... they also start to pluck their feathers as well - 'lining the nest'.... when they decide that they have enough eggs to sit on, that's it, they won't be moved.... I drag mine off the eggs once each day so they can have a drink, a bit to eat & a poo - holding my nose! 
Depending on what hen the chick has come from, I can't tell which are hens & which are roosters - I have to wait a while until they grow up a little bit, but our rooster boys will get one shot at the bird sales, then it's into the pot for them. The hens also will have a shot at the bird sales but they might well get 2 chances before heading to the kitchen! 
Out of the young ones, I have to say it's the muscovy ducklings that are the cutest! And they love cuddles too  
